A growth curve, based on cytopathic effect cpe, immunofluorescence if, haemadsorption. It is widespread in north american and european pig populations, but clinical disease is rare. The species porcine hemagglutinating encephalomyelitis virus hev, a member of genus coronavirus, family coronaviridae, can be isolated from the respiratory tract of normal pigs, and is present worldwide. It mainly gives rise to encephalomyelitis or vomiting and wasting disease in piglets. Coronavirus canine, fip, and fecov flashcards quizlet. An acute encephalomyelitis hev in piglets up to 47 days of age, a vomiting and wasting disease vwd. Porcine hemagglutinating encephalomyelitis virus phev is a singlestranded, positivesense rna virus, belonging to the coronaviridae family of the nidovirales order. The porcine hemagglutinating encephalomyelitis virus phev is classified as a member of genus betacoronavirus, family coronaviridae, subfamily cornavirinae, and order nidovirales. An experimental model of neurodegenerative disease based on. The species porcine hemagglutinating encephalomyelitis virus hev, a member. Porcine hemagglutinating encephalomyelitis virus betacoronavirus 1 is a species of coronavirus which infects humans and cattle.
Porcine haemagglutinating encephalomyelitis virus phev is the main causative agent of porcine coronavirusassociated disease, which is characterized by encephalomyelitis and involves the central nervous system. Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus. It is caused by coronavirus porcine hemagglutinating encephalomyelitis virus phev, which is widespread in the pig population in north america and europe. Coronaviruslike particles were observed in the homogenized tissue suspensions of the brain of dead piglets by electron microscopy, and a wild phev strain was isolated, characterized, and designated as. Porcine hemagglutinating encephalomyelitis phe is an infectious disease primarily affecting pigs under 3 weeks of age, causing vomiting, exhaustion, and obvious neurological symptoms. Frontiers induction of atypical autophagy by porcine. Porcine hemagglutinating encephalomyelitis virus phev is a member of the genus coronavirus, family coronaviridae, that mainly causes vomiting and wasting disease vwd andor encephalomyelitis in piglets under 3 weeks old 1, 2. We describe an outbreak of vomiting, wasting, and encephalomyelitis syndrome in piglets in argentina, caused by porcine hemagglutinating encephalomyelitis coronavirus phecov infection. Porcine hemagglutinating encephalomyelitis virus and respiratory disease in exhibition swine, michigan, usa, 2015.
Porcine hemagglutinating encephalomyelitis virus strain 67n hev67n. Comparative epidemiology of porcine circovirus type 3 in. Vomiting and wasting disease, coronaviral encephalomyelitis. Virus induced cytoskeletal dynamics are thought to be closely related to this type of nerve damage. Porcine hemagglutinating encephalomyelitis virus strain 67n hev67n status. Porcine respiratory coronavirus prcov is a minimally pathogenic virus that arose in the mid1980s by deletion and point mutation of the transmissible gastroenteritis virus tgev genome.
Does circulating antibody play a role in the protection of. Previous studies have demonstrated that the virus has a strong tropism for the upper respiratory tract. First detection and characterisation of porcine hemagglutinating. Its genetic material is the largest of all rna viruses and. However, the circulating status of pcv3 in pigs with other clinical presentations. Porcine hemagglutinating encephalomyelitis disease causes vomiting and wasting disease in pigs younger than 2 weeks old. Diagnosis was made by epidemiologic factors, pathologic features, immunohistochemistry, reverse transcriptionpcr, and genomic sequencing. The causal coronavirus, porcine hemagglutinating encephalomyelitis virus phev, is of a single antigenic type, and it grows in several types of porcine cell cultures, in which it causes syncytia.
Porcine hemagglutinating encephalomyelitis phe is an infectious disease primarily affecting pigs under 3 weeks of age, causing vomiting, exhaustion, and neurological symptoms cartwright et al. Porcine hemagglutinating encephalomyelitis virus phev is a highly neurovirulent coronavirus that invades the central nervous system in piglets. The disease was first reported in 1958, and the virus was first isolated from the piglets in 1962 3, 4. The evidence of porcine hemagglutinating encephalomyelitis virus induced nonsuppurative encephalitis as the cause of death in piglets zi li 1, wenqi he, yungang lan 1, kui zhao, xiaoling lv, huijun lu2, ning ding 1, jing zhang, junchao shi 1, changjian shan and feng gao1. Porcine hemagglutinating encephalomyelitis virus rabbit coronavirus a genus of the family coronaviridae which causes respiratory or gastrointestinal disease in a variety of vertebrates.
Reference sequences obtained from genbank are indicated by strain name and accession number. Pdf evolutionary history of the closely related group 2. Porcine hemagglutinating encephalomyelitis virus phev is a highly neurovirulent coronavirus that. Porcine hemagglutinating encephalomyelitis virus phev displays neurotropism in mice and neural cells are its targets for viral progression. Find specific details on this topic and related topics from the msd vet manual. No commercial phev vaccines are available, and neonatal protection from phevassociated disease is presumably dependent on. Scale bar represents nucleotide substitutions per site. Porcine hemagglutinating encephalomyelitis virus an.
Genomic comparison of 10 porcine hemagglutinating encephalomyelitis virus phev strains from fairs in michigan, indiana, and ohio, usa, 2015, to 3 nonphev coronavirus cov strains from genbank bovine cov kakegawa, accession no. European and american isolates of prcov differ, and apparently developed independently from tgev. Porcine hemagglutinating encephalomyelitis synonyms. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Geneexpression patterns in the cerebral cortex of mice. Protection from the disease could be provided through lactogenic. Porcine hemagglutinating encephalomyelitis, a viral disease of young pigs, is characterized by. Evolutionary history of the closely related group 2 coronaviruses. As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Antonyms for porcine hemagglutinating encephalomyelitis.
Phev porcine hemagglutinating encephalomyelitis virus. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. To gain insight into the interaction between the virus and host cells, changes in global gene expression. During the summer of 2015, an ongoing influenza a virus iav surveillance program targeting exhibition swine was expanded to include 14 michigan fairs. Porcine hemagglutinating encephalomyelitis virus phev, a lesserresearched. Porcine hemagglutinating encephalomyelitis virus phev, a lesserresearched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs. Porcine hemagglutinating encephalomyelitis virus phev is a singlestranded, positivesense, rna virus in the genus betacoronavirus, family coronaviridae. The disease is caused by phe coronavirus phecov, which comprises a single strain and is the only known neurotropic cov affecting pigs.
Testing ruled out influenza a virus and identified porcine hemagglutinat ing encephalomyelitis virus as the cause of influenzalike illness in the affected swine. It is caused by coronavirus porcine hemagglutinating encephalomyelitis virus phev, which is widespread in the pig population in north america and europe, but its clinical disease is rare. Coronavirus is a positivestranded singlestranded rna infection with an envelope of around 80 to 120 nm in width. Porcine hemagglutinating encephalomyelitis virus, bovine coronavirus, and human coronavirus oc43. Little is known about the molecular mechanisms of brain injury caused by phev.
Synonyms for porcine hemagglutinating encephalomyelitis in free thesaurus. An immunochromatographic strip was developed for the detection of an antibody against porcine hemagglutinating encephalomyelitis virus phev. Porcine circovirus type 3 pcv3, as an emerging circovirus species, was reported to be widely circulating in the united states, china, south korea and poland. The disease is caused by porcine hemagglutinating encephalomyelitis virus phev, a coronavirus. This study reports some cultural characteristics of the vw 572 strain of hemagglutinating encephalomyelitis virus hev in primary pig kidney ppk cells, primary pig testicle ppt cells, secondary pig thyroid spth cells and the cell lines pk.
Porcine hemagglutinating encephalomyelitis, a viral disease of young pigs, is characterized by vomiting, constipation, and anorexia and results either in rapid death or chronic emaciation vomiting and wasting. The rcsb pdb also provides a variety of tools and resources. The porcine hemagglutinating encephalomyelitis virus phev is classi. Pdf porcine hemagglutinating encephalomyelitis virus.
Porcine hemagglutinating encephalomyelitis virus phev is a singlestranded, positivesense rna virus. Hemagglutinating encephalomyelitis swine diseases manual. Pdf porcine hemagglutinating encephalomyelitis virus and. Health, general encephalomyelitis fairs exhibitions influenza lung diseases. Consistent protection from clinical disease in suckling pigs is dependent on herd endemicity. Five coronavirusassociated diseases have been reported in swine. Overview of porcine hemagglutinating encephalomyelitis. Like other coronaviruses covs, phev is enveloped and has prominent surface glycoproteins that protrude from the membrane. The evidence of porcine hemagglutinating encephalomyelitis. Porcine hemagglutinating encephalomyelitis virus an overview.
This is because most sows that have been infected, so they are immune, and pass antibodies to their piglets through colostrum, which protects them during the period when they are vulnerable. Phev was one of the first swine coronaviruses identified and isolated, and the only known neurotropic virus that affects pigs. An acute outbreak of porcine hemagglutinating encephalomyelitis virus phev infection in piglets, characterized with neurological symptoms, vomiting, diarrhea, and wasting, occurred in china. Phev shares the same genomic organization, replication strategy, and expression of viral proteins as other nidoviruses. The porcine hemagglutinating encephalomyelitis virus phev is the. Phev was first isolated from the brains of suckling piglets with encephalomyelitis over five decades ago. Yungang lan, kui zhao, gaili wang, bo dong, jiakuan zhao, bo tang, huijun lu, wei gao, lingzhu chang, zhao jin, feng gao and wenqi he, porcine hemagglutinating encephalomyelitis virus induces apoptosis in a porcine kidney cell line via caspasedependent pathways, virus research, 176, 12, 292, 20.
The porcine hemagglutinating encephalomyelitis virus phev is the causative agent of neurological andor digestive disease in pigs. Porcine hemagglutinating encephalomyelitis virus phev is a singlestranded, positivesense rna coronavirus and is classified as a member of the coronaviridae family andries and pensaert, 1981. The authors studied the cultural characteristics of the vw 572 strain of haemagglutinating encephalomyelitis virus hev in primary pig kidney ppk cells, primary pig testis ppt cells, secondary pig thyroid spth cells and the cell lines pk15 pig kidney, sk6 swine kidney and st swine testis. Haemagglutinating encephalomyelitis, vomiting and wasting disease, vwd, hev. Haemagglutinating encephalomyelitis vomiting and wasting disease is a viral disease of pigs caused by a coronavirus. No commercial phev vaccines are available, and neonatal protection from. Propagation of hemagglutinating encephalomyelitis virus in. Porcine hemagglutinating encephalomyelitis virus phev is a. Colloidal goldlabeled rabbit antipig immunoglobulin. Porcine hemagglutinating encephalomyelitis virus hev canine coronavirus ccov bovine coronavirus bcov mouse hepatitis virus mhv in breeding colonies severe acute resp syndrome virus sarscov zoonotic middle east respiratory syndrome virus merscov zoonotic. Phylogenetic tree constructed on the basis of the ns2 gene of porcine hemagglutinating encephalomyelitis virus phev strains, bovine cov, human enteric cov, and white tail deer cov. Also, motor disorders due to acute encephalomyelitis hemagglutinating encephalomyelitis are often seen during field outbreaks. Development and evaluation of an immunochromatographic. Previous studies revealed that pcv3 was mainly concentrated in sick animals with respiratory disease, skin disease, reproductive disorders and so on.
1571 477 1169 1052 135 1634 1370 1625 1011 913 345 1500 1147 1286 135 635 1452 1509 1535 1097 731 566 1092 1308 48 1107 396 1023 1001 317 146 737 2 1485 881 96 4 177 83 1093 463